別品名 |
Ser 1 antibody, AGS antibody, AHD antibody, AWS antibody, CD 339 antibody, CD339 antibody, CD339 antigen antibody, Headturner antibody, HJ1 antibody, Htu antibody
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抗原部位 |
a.a.110-125
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse
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GENE ID |
182
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Accession No.(Gene/Protein) |
P78504
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Gene Symbol |
JAG1
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]27170649
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
(a) Representative confocal microscopy shows CD44, CD24 and cleaved notch (NICD) in a population of drug naive MDA MB 231. Yellow arrows indicate CD44HiCD24Lo (M) population of cells and the white arrows indicate the CD44HiCD24Hi (E/M) cells. Histogram (right panel) shows quantification of NICD in the distinct phenotype populations (M versus E/M). N = 3 biological replicates. (b) Schematic describes the experimental protocol to generate drug tolerant cells (DTCs) parental MDA MB 231 cells were treated with docetaxel at 100 nM (20x the IC50) and subsequently selected by substrate re attachment and acute population outgrowth. (c) Representative confocal microscopy shows CD44, CD24 and NICD in the MDA MB 231 parent and DTC populations. Right panel shows quantification of fluorescence intensity of each signal determined by at least 25 individual fields. N = 3 biological replicates. (d) Representative confocal microscopy shows Jagged and Delta expression in MDA MB 231 parent and DTC. DAPI nuclear stain (blue). N = 3 biological replicates. Figure provided by CiteAb. Source: J R Soc Interface, PMID: 27170649.
Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Rockland's Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.
Immunofluorescence microscopy using Rockland's Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.? Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzamine (blue).? Personal Communication.? Aihua Ma, Univdersity of Cardiff.
Western Blot of Rabbit Anti-Jagged 1 Antibody. Lane 1: Opal Prestained MW marker (p/n MB-210-0500). Lane 2: Mouse Liver Whole Cell Lysate [10ug] (p/n W10-000-T020). Lane 3: Human Liver Whole Cell Lysate [10ug]. Lane 4: Human Lung Whole Cell Lysate [10ug]. Primary Antibody: Anti-Jagged 1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase Conjugated (p/n 611-103-122) at 1:70000 for 30mins at RT. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 1hr RT. Predicted Molecular Weight: 113kDa. Exposure: 30 sec.
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(a) Representative confocal microscopy shows CD44, CD24 and cleaved notch (NICD) in a population of drug naive MDA MB 231. Yellow arrows indicate CD44HiCD24Lo (M) population of cells and the white arrows indicate the CD44HiCD24Hi (E/M) cells. Histogram (right panel) shows quantification of NICD in the distinct phenotype populations (M versus E/M). N = 3 biological replicates. (b) Schematic describes the experimental protocol to generate drug tolerant cells (DTCs) parental MDA MB 231 cells were treated with docetaxel at 100 nM (20x the IC50) and subsequently selected by substrate re attachment and acute population outgrowth. (c) Representative confocal microscopy shows CD44, CD24 and NICD in the MDA MB 231 parent and DTC populations. Right panel shows quantification of fluorescence intensity of each signal determined by at least 25 individual fields. N = 3 biological replicates. (d) Representative confocal microscopy shows Jagged and Delta expression in MDA MB 231 parent and DTC. DAPI nuclear stain (blue). N = 3 biological replicates. Figure provided by CiteAb. Source: J R Soc Interface, PMID: 27170649.
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メーカー |
品番 |
包装 |
RKL
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200-401-698S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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