Anti Jagged-1

(a) Representative confocal microscopy shows CD44, CD24 and cleaved notch (NICD) in a population of drug naive MDA MB 231. Yellow arrows indicate CD44HiCD24Lo (M) population of cells and the white arrows indicate the CD44HiCD24Hi (E/M) cells. Histogram (right panel) shows quantification of NICD in the distinct phenotype populations (M versus E/M). N = 3 biological replicates. (b) Schematic describes the experimental protocol to generate drug tolerant cells (DTCs) parental MDA MB 231 cells were treated with docetaxel at 100 nM (20x the IC50) and subsequently selected by substrate re attachment and acute population outgrowth. (c) Representative confocal microscopy shows CD44, CD24 and NICD in the MDA MB 231 parent and DTC populations. Right panel shows quantification of fluorescence intensity of each signal determined by at least 25 individual fields. N = 3 biological replicates. (d) Representative confocal microscopy shows Jagged and Delta expression in MDA MB 231 parent and DTC. DAPI nuclear stain (blue). N = 3 biological replicates. Figure provided by CiteAb. Source: J R Soc Interface, PMID: 27170649.