別品名 |
Actin Antibody, Loading Control Antibody, beta actin, β actin, anti-beta actin antibody
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抗原部位 |
N-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Mouse
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抗体クラス |
IgG2aλ
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クローン |
3H2.E4.E12
|
交差種 |
Human Mouse
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GENE ID |
11461, 60
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Accession No.(Gene/Protein) |
NP_001092, NP_031419
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Gene Symbol |
Actb
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]27191820, 24410055
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Mouse anti Beta Actin N Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N Term antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western Blot of Mouse anti-beta Actin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Raji Lysate (p/n W09-001-368). Lane 10: Ramos Lysate (p/n W09-000-GK4). Lane 11: PC-12 (p/n W12-001-GL9). Load: 10 μg per lane. Primary antibody: beta-Actin antibody at 1:1,000 o/n at 4C. Secondary antibody: Peroxidase mouse secondary (p/n 610-403-C46) at 1:40,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 42 kDa for beta-Actin.
Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody. Lane 1: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 2: NIH/3T3 Whole Cell Lysate (p/n W10-000-358). Load: 10 ug per lane. Primary antibody: N-Term Beta Actin Monoclonal Antibody at 1.13ug/mL overnight at 4C. Secondary antibody: HRP mouse secondary antibody (p/n 610-403-C46) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa for N-Term Beta Actin.
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Immunohistochemistry of Mouse anti Beta Actin N Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N Term antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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メーカー |
品番 |
包装 |
RKL
|
200-301-E69S
|
25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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