Anti Cdk5  

Western Blot of Anti-CDK5 (Mouse) Antibody. Lane 1: rat caudate lysate. Lane 2: none. Load: 10 μg per lane. Primary antibody: CDK5 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800^TM mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~ 28kDa/~28kDa for Cdk5 protein. Other band(s): none.

F)？Immunoblot of Cdk5 pathway components in fibroblasts and PanNET cells. Fig 1. PMID:？34862365

PKA regulatory subunit RIIβ phosphorylation at Thr69 by Cdk5. A), tissue distribution of R subunits and Cdk5 in rat peripheral and central nervous system tissues. For RIIβ blot,？dotted lines？denote the lane loaded for skeletal muscle was moved to correspond to the loading order for other blots.？B), co-immunostain of RIIβ and AKAP150 in rat ventral striatum.？C), colocalization of Cdk5 and RIIβ in dissociated rat striatal neurons.？D), immunofluorescent colocalization of Cdk5 and RIIβ in rat striatum with nuclear DAPI counterstain.？E),？time-course？in？vitro？phosphorylation of RIIβ by Cdk5 (autoradiogram,？32P,？top; Coomassie stain CBB,？middle) with accompanying ESI-Qq TOF MS/MS spectrum (bottom) of the tryptic peptide shown encompassing phospho-Thr69 (pT, ion peak y4) positively identifying the site of RIIβ phosphorylation by Cdk5.？F),？in？vitro？phosphorylation of WT？versus？T69A RIIβ by Cdk5 with stoichiometry.？G), immunostain of phospho-Thr69 RIIβ (brown,？top right, and？bottom？two images) with nuclear counterstain (purple) in rat striatum.？Top left？image shows background with secondary antibody alone. AKAP, A-kinase anchoring protein; Cdk5, cyclin-dependent kinase 5; PKA, cAMP-dependent protein kinase. Fig 1. PMID:？35835216

(F) Immunoblots of Cdk5, p25/p35 in WT and？SDHB？KO cells with quantitation.(J) Quantitative immunoblotting of Cdk5/p35/p25 levels in tumor lysates derived from WT (n = 4) and KO (n = 6) xenografts. Values are means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001; n.s., non-significant compared by t test and/or one-way ANOVA. Fig 2. PMID:？35977518

(D) Immunoblots showing expression of p25-GFP, Cdk5, TH, and ChrA in tissue lysates derived from p25-ON or WT adrenal glands. Fig 4. PMID:？35977518

(E) Hematoxylin eosin (HE) and immunostains of Cdk5 and p35/p25 in human PC, scale bar, 50 μm.(F) Histological assessment of Cdk5 and p35/25 in tissue microarray sections of adrenocortical adenoma (ACA, n = 40) and PC (n = 30); quantification presented as optical density; *p < 0.05, **p < 0.01, ***p < 0.001 compared using t test with Welch’s correction; n.s., non-significant. Fig 3. PMID:？35977518

(E) Histological assessment of NE markers in WT adrenals or PC tissue sections, scale bar 100 μM. Fig 4. PMID:？35977518