| 別品名 |
CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, Interleukin 10 antibody, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Serum
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
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| GENE ID |
3586
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| Accession No.(Gene/Protein) |
P22301
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| Gene Symbol |
IL-10
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Elias A. Said et al. (2009) PD-1 Induced IL10 Production by Monocytes Impairs T-cell Activation in a Reversible Fashion. Nature Medicine. 2010; 452-9.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland Anti IL 10 whole antiserum (109 401 312) was tested against recombinant IL10. Recombinant IL 10 was run on a 4 20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA TBS for 1 Hour 4C and probed with (p/n 109 401 312) diluted 1:200 in 3% BSA TBS o/n 4C. Primary antibody was detected with HRP Gt anti Rb (611 103 122) Lot#21231 1:20,000 in MB 070 1 Hour 4C using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Biorad. Other detection systems will yield similar results.
Western Blot of Rabbit anti-IL-10 antibody. Lane 1: Recombinant Human IL-10. Load: 50 ng per lane. Primary antibody: IL-10 antibody at 1:500 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for IL-10.
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Rockland Anti IL 10 whole antiserum (109 401 312) was tested against recombinant IL10. Recombinant IL 10 was run on a 4 20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA TBS for 1 Hour 4C and probed with (p/n 109 401 312) diluted 1:200 in 3% BSA TBS o/n 4C. Primary antibody was detected with HRP Gt anti Rb (611 103 122) Lot#21231 1:20,000 in MB 070 1 Hour 4C using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Biorad. Other detection systems will yield similar results.
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| メーカー |
品番 |
包装 |
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RKL
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109-401-312S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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