Anti NFkB p65  

Immunohistochemistry of NFKB p65 (Rel A) antibody. Tissue: lymphocytes and germinal center cells of the human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: NFKB p65 (Rel A) antibody at 1:400. Secondary antibody: Peroxidase goat anti-rabbit at (p/n 611-103-122) 1:10,000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Moderate positive nuclear or cytoplasmic staining was observed in lymphocytes and germinal center cells of the tonsil.

P5 inhibits NF-κB nuclear translocation in？P.？acnes-infected HK cells.The intracellular distribution of NF-κB was determined by immunofluorescent labeling of NF-κB p65 (green), while nuclei were Hoechst stained (blue): (A) untreated HK cells; (B) HK cells treated with？P.？acnes; (C) HK cells treated with？P.？acnes？plus P5; HK cells treated with？P.？acnes？plus P4 (D).Fig 5. PMID:？26197393.

Western Blot of Rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell lysate (p/n W09-000-364). Lane 2: HeLa cell lysate (p/n W09-000-364). Load: 35 μg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None.

Western Blot of Rabbit anti-NFkB antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lysate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 10 μg per lane. Primary antibody: NFkB antibody at 1:500 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 65 kDa for NFkB.

NF-κB complexes are recruited to the？EIF4E？promoter and promote transactivation. BJAB cells were stimulated with PMA for 0, 1 or 2 hours and subjected to chromatin immunoprecipitation using antibodies specific for (A) NFKB p65？(p/n 100-4165), (B) cRel, (C) p300？transcription factor (p/n 100-301-176)？and phosphorylated Pol II (Ser2/Ser5)？(D). Data were normalized to IgG control and represented as fold enrichment with respect to untreated cells. Error bars represent standard deviations from triplicate measurements of a representative experiment. Figure 3. PMID:？23467026

Constitutively active NF-κB regulates eIF4E expression in KM-H2 cells.(A)？EMSA analysis of KM-H2 nuclear extracts using probes corresponding to the κB3 and κB4 sites. Supershift analysis using antibodies against NFKB p65 (p/n 100-4165), NFKB p50 p/n 100-4164), cRel and IgG control as well as competition with consensus cold probe (CCP) were done. Protein/DNA complexes are indicated by arrows and supershifted complexes by arrowheads. Free probe is also shown.？Figure 5. PMID:？23467026

Western Blot and ChIP results using NF-κB. Effects of PKC, p38MAPK or PI3K pathway inhibition on NF-κB subunits nuclear translocation？in vitro: THP-1 cells were preincubated for 45 min in the absence or presence of the PKC, p38MAPK or PI3K inhibitor before treatment with NE for 15 min. (A) Nuclear extracts of THP-1 cells were immunoblotted with antibodies to p50, p65 (p/n 100-4165), cRel (p/n 100-4166) and histone-1. The signal intensity was evaluated and is summarized in the bargraphs. Statistical significant differences are indicated (*p？< 0.05). Data represent the mean ± SD (n？= 5). (B) The recruitment of p50, p65 and cRel-subunits to the？ICAM-1？promoter was determined using ChIP-assays.？Fig 3. PMID:？23114885.