Anti NOTCH 2

Rockland's anti NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Western Blot of Rabbit Anti-Notch 2 N-Term Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 3: MOLT-4 Whole Cell Lysate (p/n W09-001-GK4). Lane 4: K-562 Whole Cell Lysate (p/n W09-001-GJ7). Lane 5: Human Kidney Lysate. Load: 20μg lysate/lane. Primary Antibody: Anti-NOTCH 2 at 1:1000 for 1 hr at RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Blocking: 5% BLOTTO. Expected MW: ~86kDa.

Western Blot of Rabbit anti-NOTCH2 (Cleaved N terminal) antibody. Lane 1 Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: U-251 Lysate (p/n W09-001-GY4). Lane 3: PC-3 Lysate (p/n W09-001-GV6). Lane 4: MCF-7 Lysate (p/n W09-000-360). Lane 5: Hep-G2 Lysate (p/n W09-001-GJ5). Lane 6: A431 Lysate (p/n W09-000-361). Lane 7: A549 Lysate (p/n W09-001-372). Lane 8: J774A.1 Lysate (p/n W10-001-GX3). Lane 9: C2C12 Lysate (p/n W10-001-GL7). Lane 10: Mouse Embryonic Fibroblast Lysate (p/n W10-001-371). Lane 11: NIH/3T3 Lysate (p/n W10-000-358). Lane 12: PC-12 Lysate (p/n W12-001-GL9). Lane 13: Rat Brain Lysate (p/n W12-000-T077). Lane 14: Mouse Brain Lysate (p/n W10-000-T004). Lane 15 Marker: Opal Pre-stained Ladder. Load: 10 ug of lysate/lane. Primary antibody: NOTCH2 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted size: ~86kDa in human cell lines (2-7) for NOTCH2.