| 別品名 |
Stripping solution, Western Blot stripping buffer, western blot reprobing buffer, bound antibody removal buffer
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| 適用 |
Western Blot
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| 形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot comparison of RevitablotTM Western Blot Stripping Buffer. Lane 1: 70 ug HeLa whole cell lysates. Lane 2: 10 ug HeLa whole cell lysates. Blot A first probed with: Primary antibody: Rabbit Anti STAT5 (p/n 600 401 A44) at a dilution of 1:1,000 overnight at 4C. Secondary antibody: HRP Goat anti rabbit (p/n 611 103 122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX 110). Membranes were then incubated with Revitablot and a competitor's product for 20 minutes at room temperature, followed by re blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti tubulin (p/n 600 401 880) at a dilution of 1:1,000 overnight at 4C. Secondary antibody: HRP Goat anti rabbit (p/n 611 103 122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX 110).
Western Blot comparison of RevitablotTM Western Blot Stripping Buffer using Fluorescent labeled Primary Antibodies. Lane 1: 7.5 ug HCT-116 cell lysates. Lane 2: 22.5 ug HCT-116 cell lysates. Lane 3: 15.0 ug HCT-116 cell lysates. Block: 5% BLOTTO for 1 hr at room temperature. Blot A first probed with: Primary antibody: Mouse anti-AKT pS473 (p/n 200-343-268) at a dilution of 1:1,000 overnight at 4C. Imaging: 650nm. Membranes were then incubated with RevitablotTM 5 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-beta actin DyLight 488 Conjugated at a dilution of 1:1,000 for 4 hours at room temperature. Imaging: 488nm and 650nm.
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Western Blot comparison of RevitablotTM Western Blot Stripping Buffer. Lane 1: 70 ug HeLa whole cell lysates. Lane 2: 10 ug HeLa whole cell lysates. Blot A first probed with: Primary antibody: Rabbit Anti STAT5 (p/n 600 401 A44) at a dilution of 1:1,000 overnight at 4C. Secondary antibody: HRP Goat anti rabbit (p/n 611 103 122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX 110). Membranes were then incubated with Revitablot and a competitor's product for 20 minutes at room temperature, followed by re blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti tubulin (p/n 600 401 880) at a dilution of 1:1,000 overnight at 4C. Secondary antibody: HRP Goat anti rabbit (p/n 611 103 122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX 110).
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| メーカー |
品番 |
包装 |
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RKL
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MB-085-0500
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500 ML
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
常温
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