Anti β Actin

Immunohistochemistry of Mouse anti Beta Actin N Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N Term antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Western Blot of Mouse anti-beta Actin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Raji Lysate (p/n W09-001-368). Lane 10: Ramos Lysate (p/n W09-000-GK4). Lane 11: PC-12 (p/n W12-001-GL9). Load: 10 μg per lane. Primary antibody: beta-Actin antibody at 1:1,000 o/n at 4C. Secondary antibody: Peroxidase mouse secondary (p/n 610-403-C46) at 1:40,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 42 kDa for beta-Actin.

Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody. Lane 1: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 2: NIH/3T3 Whole Cell Lysate (p/n W10-000-358). Load: 10 ug per lane. Primary antibody: N-Term Beta Actin Monoclonal Antibody at 1.13ug/mL overnight at 4C. Secondary antibody: HRP mouse secondary antibody (p/n 610-403-C46) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa for N-Term Beta Actin.