Agitoxin-2-Cys-TAMRA

ManufacturerBioassayAlomone Labs Agitoxin-2-Cys-TAMRA retains the inhibitory characteristics of the wild type toxin. - A. KV？current inhibition by？Agitoxin-2？(#STA-420), (100nM, pink) and？Agitoxin-2-Cys-TAMRA？(#RTA-420-T), (100nM, blue) in KV1.3 transfected HEK-293 cells. Top: Time course of KV1.3 current amplitude is presented. Currents were elicited from holding potential of -100 mV by 200 ms voltage pulses to +50 mV, every 10 seconds. Bottom: Superimposed traces taken from the same experiment shown on top, before (black) or during toxin perfusion (pink or blue).B. Dose response of KV1.3 current inhibition in？Xenopus？oocytes for Agitoxin-2, Agitoxin-2-Cys-TAMRA and Agitoxin-2-Cys. Top: time course of current amplitude, upon toxin perfusion. Currents were elicited from resting potential of -100 mV, by a 100 ms test pulse to 0 mV, delivered every 10 seconds. The bars above the plot represent periods of toxin perfusion, for each toxin sequential applications of the following concentrations: 1, 10, 50 and 100 nM, where the toxin species is indicated. Middle: Superimposed traces representing currents under control conditions (black) and during perfusion of 100 nM toxin: Agitoxin-2-Cys-TAMRA (orange), Agitoxin-2 (blue) and Agitoxin-2-Cys (green). Bottom: Dose response of toxins on current inhibition (n = 3 for each toxin, mean and SD). See inset for color coding.