NUCLEAR-ID^(R) Red DNA Stain  
Nuclear-ID (商標名)

NUCLEAR-ID® Red DNA stain Flow Cytometry％％Relative costs of using NUCLEAR-ID Red DNA in comparison to competitor dye in various applications: (A) Imaging (visualization), (B) Nucleated Cell Gating (flow cytometry) and (C) Live Cell Cycle analysis using flow cytometry. Dilutions can vary depending on cell strain and cell concentration.Notes:Assumes staining of a 100 µL staining volumeAssumes staining of a 500 µL cell suspension volumeAssumes a staining of 500 µL cell suspension volume

NUCLEAR-ID® Red DNA stain concentration％％NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or with a competitor’s dye at an equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min and 24h. Results show that 4000x NUCLEAR-ID Red DNA Stain was required for visualization of the dsDNA, while equivalent to 2000x was required for the competitor’s dye. At 24h, the competitor’s dye intensity and cell growth were dramatically reduced at the 2000x equivalent final concentration. At the same time point, 2000x of NUCLEAR-ID Red DNA Stain did not affect cell growth or fluorescent intensity. The NUCLEAR-ID Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs.

NUCLEAR-ID® Red DNA stain rev％％NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or a competitor’s dye at the equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min.

NUCLEAR-ID® Red DNA stain spectra