| 検出系 |
蛍光
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| 使用目的 |
生きた細胞における多剤耐性フェノタイプの機能検出とプロファイリングのために設計されています。
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| 構成内容 |
・eFluxx-IDTM Green Detection Reagent
・MDR1 Inhibitor (Verapamil)
・MRP Inhibitor (MK-571)
・BCRP Inhibitor (Novobiocin・ropidium Iodide
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1: Detect activity of all three major ABC transporter proteins. ABC transporter protein activity was evaluated in CHO K1 cells by flow cytometry using eFluxx-IDR Green (top), Gold (middle), or Calcein AM (bottom) dyes. Treatment with specific inhibitors of ABC Transporter proteins (shaded histograms) induces retention of dye within cells relative to untreated cells (lined histograms). The difference in mean fluorescence intensity (MFI) is an indication of the corresponding protein activity, as shown by MAF scores [multidrug resistance activity factors], a quantitative measurement of multidrug resistance. Higher MAF scores are a result of superior specificity of eFluxx-ID dyes to specific inhibitors. Calcein AM (a common probe for MDR assays), is unable to detect BCRP activity.
Figure 2: Profiling of ABC transporter activity by known inhibitors was assessed in CHO K1 cells using eFluxx-IDR Green and eFluxx-IDR Gold dyes. Cells were incubated for 5 min at 37°C with general MDR Inhibitor (far left column) or transporter-specific inhibitors included in the kit. Cells were then loaded with the indicated dye for 30 min at 37°C and immediately analyzed by flow cytometry. Inhibitors used: 5 μM Cyclosporin A (general MDR inhibitor); 20 μM Verapamil (specific P-gp inhibitor); 0.05 mM MK-571 (specific MRP inhibitor); 0.05 mM Novobiocin (specific BCRP inhibitor).
Figure 3: Spectral characteristics of the eFluxx-ID Green 490/514 nm ex/em and Gold 530/570 nm ex/em reagents allows for multiplexing with other common fluorescent dyes.
Table 1: Compatibility of eFluxx-ID MDR dyes with other fluorescent dyes.
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Figure 1: Detect activity of all three major ABC transporter proteins. ABC transporter protein activity was evaluated in CHO K1 cells by flow cytometry using eFluxx-IDR Green (top), Gold (middle), or Calcein AM (bottom) dyes. Treatment with specific inhibitors of ABC Transporter proteins (shaded histograms) induces retention of dye within cells relative to untreated cells (lined histograms). The difference in mean fluorescence intensity (MFI) is an indication of the corresponding protein activity, as shown by MAF scores [multidrug resistance activity factors], a quantitative measurement of multidrug resistance. Higher MAF scores are a result of superior specificity of eFluxx-ID dyes to specific inhibitors. Calcein AM (a common probe for MDR assays), is unable to detect BCRP activity.
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| メーカー |
品番 |
包装 |
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ENZ
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ENZ-51029-K100
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
劇
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| 保存温度 |
-７０℃
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