種由来 |
Avian
|
精製度 |
Ascites
|
適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay Flow Cytometry Immunoprecipitation
|
免疫動物 |
Rat
|
抗体クラス |
IgM
|
クローン |
373E1
|
その他 |
[Background] Keratan sulfates (KSs) are sulfated polymers of N-acetyllactosamine structured by repeating (1 3)- -D-galactose-(1 4)- -D-N-acetylglucosamine units, which are generally sulfated at position C6 of the hexosamine and/or galactosamine. They are mostly covalently bound to core proteins of KS-bearing proteoglycans (PGs), but a few non-proteoglycan KS-substituted macromolecules have been described, and their attachment to protein backbones occurs primarily through an N-linkage involving glucosamine binding to an aspargine residue These are referred to type I KSs and are characteristic of the corneal ECM. KS chains may also be bound to proteins through an O-glycosidic linkage between galactosamine and a serine or threonine residue,i.e. referred to as type II KSs and highly represented in articular cartilage ECM. Phosphocan and other KS-containing PGs of the brain may also carry KS chains attached to the core protein through an alternative mannose-serine/threonine linkage. Oneof the complexities of KSs is the variable degree of chain branching (i.e. bi-antennary in the cornea and more intricate branching in skeletal KS), which together with the variable extent and positioning of the sulfate groups and the relative frequency, linkage and type of capping fucose and/or neuraminic acid residues, creates a spectrum of putative functionally diverse KS moieties. For instance, sialic acid residues may coincidently, or in an alternated fashion be present in an (1-3), (2-3)or(2-6)-linkage, and may or may not associate with (1-3)-linked fucoses [Application] Recommended use : WB, IHC(P), IP, FC, ELISA Recommended Antibody Dilutions Western blotting : 1/50 ? 1/170 Band sizing and pattern depends upon the coreprotein size of the molecule (mostly proteoglycans) bearing keratin sulfate chains. If isolated chains are separated by PAGE, the banding patern appears as a smear and the approximate molecular weights of the bands depend upon the mass and polydispersityof the chains. Immunohistochemistry : 1/50 to 1/150 ELISA, 1/100 - 1/500 Staining Pattern Ubiquitously expressed through the body of vertebrates and expressed early on during embryogenesis around the notochord. Up-regulated in manytumourcells of epithelial origin and within the tumour stroma. Primary diagnostic marker of papillary type thyroid carcinoma.
|
※サムネイル画像をクリックすると拡大画像が表示されます。
モノクローナル抗体373E1を用いた Keratan Sulfateの組織染色図 (左) ヒト腎糸球体の蛍光免疫染色 (右) ヒト膵臓ランゲルハンス島パラフィン切片の免疫染色
|
|
|
メーカー |
品番 |
包装 |
CAC
|
PRPG-KS-M01
|
100 UL
|
※表示価格について
販売状況 |
品番変更、サイズ変更
|
当社在庫 |
なし
|
保存温度 |
-20℃
|
メーカー情報 |
メーカー名 |
コスモ・バイオ株式会社
|
略号 |
CAC
|
|