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The data and subsequent graph were obtained after performing a cytokine ELISA for Myeloperoxidase. Each known sample concentration was assayed in triplicate. The OmniKineTM Human Myeloperoxidase ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Myeloperoxidase proteins within the range of 63-4000 pg/ml.
Three concentrations of recombinant Human Myeloperoxidase was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human Myeloperoxidase recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent NM33 for serum and/or plasma samples and Assay Diluent LN28 for RPMI 10% FBS.
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