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The data and subsequent graph were obtained after performing a cytokine ELISA for Endostatin. Each known sample concentration was assayed in triplicate. The OmniKineTM Human Endostatin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Endostatin proteins within the range of 63-4000 pg/ml.
Three concentrations of recombinant Human Endostatin was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human Endostatin recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum samples and Assay Diluent 1TD for RPMI 10% FBS.
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