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The data and subsequent graph were obtained after performing a cytokine ELISA for BDNF. Each known sample concentration was assayed in triplicate. The OmniKineTM Human BDNF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human BDNF proteins within the range of 47-1500 pg/ml.
Three concentrations of recombinant Human BDNF was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human BDNF recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent NM33 for serum and/or plasma samples and Assay Diluent LN28 for RPMI 10% FBS.
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