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The data and subsequent graph were obtained after performing a cytokine ELISA for IL-1alpha. Each known sample concentration was assayed in triplicate. The OmniKineTM Murine IL-1alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-1alpha proteins within the range of 16-2000 pg/ml.
Three concentrations of recombinant Mouse IL-1alpha was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Mouse IL-1alpha recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
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