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The data and subsequent graph were obtained after performing a cytokine ELISA for TSLP. Each known sample concentration was assayed in triplicate. The OmniKineTM Human TSLP ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TSLP proteins within the range of 16-2000 pg/ml.
Three concentrations of recombinant Human TSLP was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human TSLP recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
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