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The data and subsequent graph were obtained after performing a cytokine ELISA for PLGF. Each known sample concentration was assayed in triplicate. The OmniKineTM Human PLGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human PLGF proteins within the range of 32-2000 pg/ml.
Three concentrations of recombinant Human PLGF was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human PLGF recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
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