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The data and subsequent graph were obtained after performing a cytokine ELISA for IL-17E. Each known sample concentration was assayed in triplicate. The OmniKineTM Human IL-17E ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-17E proteins within the range of 32-2000 pg/ml.
Three concentrations of recombinant Human IL-17E was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human IL-17E recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum samples and Assay Diluent 1TD for RPMI 10% FBS.
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