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The data and subsequent graph were obtained after performing a cytokine ELISA for Betacellulin. Each known sample concentration was assayed in triplicate. The OmniKineTM Human Betacellulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Betacellulin proteins within the range of 8-1000 pg/ml.
Three concentrations of recombinant Human Betacellulin was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
Human Betacellulin recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
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