別品名 |
AT550, ATTO 550, ATTO-TEC 550
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種由来 |
Rat
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標識物 |
ATTO 550
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精製度 |
Affinity Purified
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適用 |
Western Blot
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免疫動物 |
Goat
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非交差(吸収処理)種 |
Human Mouse Bovine Rabbit Chicken Sheep Goat Guinea Pig Hamster Equine
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形状 |
凍結乾燥品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
AttoTM dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO 55 Anti Aquaporin 2 antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jorg Reichwein, ATTO TEC GmbH
ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
AttoTM dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-55 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-11-12). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 12-12). Lane 2: Rat IgG F(c) Fragment (p/n 12-13). Lane 3: Rat IgG Fab Fragment (p/n 12-15). Lane 4: Rat IgM Whole Molecule (p/n 12-17). Lane 5: Rat Serum (p/n D31-5). All samples were reduced. Load: 5 ng per lane. Block: MB-7 for 3 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-11-12) 1:1, for 6 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST1) 1:4, in MB-7 for 3 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
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AttoTM dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO 55 Anti Aquaporin 2 antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jorg Reichwein, ATTO TEC GmbH
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メーカー |
品番 |
包装 |
RKL
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612-154-120S
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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