別品名 |
AT647N, ATTO 647N, ATTO-TEC 647N
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種由来 |
Rabbit
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標識物 |
ATTO 647N
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精製度 |
Affinity Purified
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適用 |
Western Blot Dot Blot
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免疫動物 |
Goat
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非交差(吸収処理)種 |
Human Mouse Rat Bovine Chicken Sheep Goat Guinea Pig Hamster Equine
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形状 |
凍結乾燥品
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参考文献 |
[DOI]10.1101/2020.04.28.066605 [Pub Med ID]24718107, 27586688, 28235049, 29686261, 29950309, 30287847, 30355502, 32075774, 34672953, 34780483, 35177616, 35307029
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland ATTO 647N conjugated anti rabbit antibody was used to detect anti Beta Actin antibody (p/n 600 401 886 lot 26928). Hela (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4 20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. Rockland ATTO 647N conjugated anti rabbit antibody (p/n 611 156 122 lot 26426C, 1:10000 in MB 070, Shown on Left and 2. Rockland HRP conjugated anti rabbit IgG (611 1322 lot 19247, 1:10000 in MB 070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Rockland Femtomax 110 super sensitive Chemiluminescent substrate using the Biorad Versa Doc Imaging System.
ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Rockland ATTO 647N conjugated anti rabbit antibody was used to detect anti-Beta Actin antibody (p/n 600-401-886 lot 26928). Hela (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. Rockland ATTO 647N conjugated anti rabbit antibody (p/n 611-156-122 lot 26426C, 1:10000 in MB-070, Shown on Left and 2. Rockland HRP conjugated anti rabbit IgG (611-1322 lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Rockland Femtomax-110 super sensitive Chemiluminescent substrate using the Biorad Versa Doc Imaging System.
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Rockland ATTO 647N conjugated anti rabbit antibody was used to detect anti Beta Actin antibody (p/n 600 401 886 lot 26928). Hela (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4 20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. Rockland ATTO 647N conjugated anti rabbit antibody (p/n 611 156 122 lot 26426C, 1:10000 in MB 070, Shown on Left and 2. Rockland HRP conjugated anti rabbit IgG (611 1322 lot 19247, 1:10000 in MB 070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Rockland Femtomax 110 super sensitive Chemiluminescent substrate using the Biorad Versa Doc Imaging System.
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メーカー |
品番 |
包装 |
RKL
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611-156-122
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500 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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