Anti IgG (H&L)  

Comparison of nephrin imaging with different conventional and super-resolution modalities. A) Widefield vs dSTORM using Atto647N. B) Confocal vs STED using Atto647N. C) Widefield vs SIM using Alexa Fluor 488. D) ExM prepared sample imaged with widefield vs e confocal using Alexa Fluor 488. The confocal ExM image furthermore shows the nucleus stained with Hoechst. Highlighted panels show ×？3 zoomed areas. Scale bars in a？c are 1？μm, in d and e 10？μm and in the zoomed in areas 5？μm (after expansion) Figure provided by CiteAb. Source: Anal Bioanal Chem, PMID: 33277998.

Dot Blot Results of Rabbit Anti-Goat IgG (H&L) Antibody (MX Hu, Ms, Rb) ATTO 647N Conjugated. Sample: Goat IgG [p/n 005-0102]. Loaded: 1) 100ng, 2) 33.33ng, 3) 11.11ng, 4) 3.7ng, 5) 1.23ng. Antibody: Anti-Goat IgG (H&L) [Rabbit] Antibody (MX3) ATTO 647N Conjugated at 1.0μg/mL for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting [p/n MB-070] for 30 mins at RT.

ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH