別品名 |
AT532, ATTO 532, ATTO-TEC 532
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種由来 |
Goat
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標識物 |
ATTO 532
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精製度 |
Affinity Purified
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適用 |
Western Blot Immuno Fluorescence Fluorescence based plate assays
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免疫動物 |
Rabbit
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交差種 |
Goat
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非交差(吸収処理)種 |
Human Mouse Rabbit
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形状 |
凍結乾燥品
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参考文献 |
[Pub Med ID]30143630
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※サムネイル画像をクリックすると拡大画像が表示されます。
ATTO (R) dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
Western Blot of Atto 532 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Atto 532 rabbit secondary antibody at 1:1,000 for 60 min at RT.Block: MB-070 for 30 min at RT.Predicted/Observed size: 50 kDa, 25 kDa for Goat IgG. Other band(s): none.
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ATTO (R) dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
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メーカー |
品番 |
包装 |
RKL
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605-453-013S
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100 UG [1.0 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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