Anti IgG (H&L)

Properties of DyLightTM conjugates.

DyLightTM dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLightTM 549 conjugate. Anti-TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.

Western Blot of Unconjugated Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.

Western Blot of DyLightTM 680 conjugated Goat anti-Chicken IgG antibody. Lane 1: Chicken IgG (p/n 003-0102). Load: 50 ng per lane. Primary antibody: none. Secondary antibody: DyLightTM 680 chicken secondary antibody at 1:5,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 72 kDa, 28 kDa for Chicken IgY. Other band(s): none.

DyLightTM 680 Fluorescence Spectra