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Properties of DylightTM Conjugates.
DyLightTM dyes can be used for two-color Western Blot detection with low background and high signal.? Anti-tubulin was detected using a DyLightTM 549 conjugate.? Anti-TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
Western Blot of Goat anti-Chicken Antibody DylightTM 488 Conjugated. Lane 1: Chicken IgG/IgY. Load: 50 ng per lane. Secondary antibody: Chicken IgG (H&L) Antibody DylightTM 488 Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 72 and 28 kDa, 72 and 28 kDa.
DyLightTM 488 Fluorescence Spectra.
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