| 別品名 |
Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
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| 種由来 |
Mouse
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Ion Exchange /Gel Filtration
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Flow Cytometry
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| 免疫動物 |
Rat
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| 抗体クラス |
IgG2aκ
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| クローン |
20B4.G10.F5
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| 交差種 |
Mouse
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| GENE ID |
16171
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| Accession No.(Gene/Protein) |
NP_034682, Q62386
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| Gene Symbol |
IL17a
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Kennedy J., Rossi D.L., Zurawski S.M., Vega F. Jr., Kastelein R.A., Wagner J.L., Hannum C.H., Zlotnik A. (1996) Mouse IL-17: a cytokine preferentially expressed by alpha beta TCR + CD4-CD8-T cells. J. Interferon Cytokine Res. 16:611-617 [PubMed: 8877732] [Abstract]
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rockland monoclonal anti IL 17A was used to detect IL 17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti CD3 antibody, and stimulated with: 5 ug/mL anti CD28, 10 ng/mL IL 1beta, 50 ng/mL mouse IL 6, 1 ng/mL TGFbeta1 and 10 ug/mL anti mouse IFN gamma over 8 10 days of culture. Cells were incubated for 15 20 minutes with addition of rat anti mouse CD4 APC at a concentration of 0.125 ug/mL, washed, fixed and permeabilized and incubated with Rockland Rat anti mouse IL 17A monoclonal Antibody (210 501 B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for Rocklands monoclonal anti mouse IL 17A antibody (210 501 B32).
Rockland monoclonal anti-IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 μg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 μg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 μg/mL, washed, fixed and permeabilized and incubated with Rockland Rat anti-mouse IL-17A monoclonal Antibody (210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C, Anti-Mouse IL-17A (RAT) Antibody (p/n 210-501-B32) was used at 1:1000 in 1% BSA-TTBS over night at 4C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody (p/n 610-4302) was diluted in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20C and imaged using the Bio-Rad VersaDocR 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
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Rockland monoclonal anti IL 17A was used to detect IL 17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti CD3 antibody, and stimulated with: 5 ug/mL anti CD28, 10 ng/mL IL 1beta, 50 ng/mL mouse IL 6, 1 ng/mL TGFbeta1 and 10 ug/mL anti mouse IFN gamma over 8 10 days of culture. Cells were incubated for 15 20 minutes with addition of rat anti mouse CD4 APC at a concentration of 0.125 ug/mL, washed, fixed and permeabilized and incubated with Rockland Rat anti mouse IL 17A monoclonal Antibody (210 501 B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for Rocklands monoclonal anti mouse IL 17A antibody (210 501 B32).
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| メーカー |
品番 |
包装 |
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RKL
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210-501-B32
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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