別品名 |
Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry
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適用 |
Western Blot
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Accession No.(Gene/Protein) |
Q9U6Y8
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Gene Symbol |
dsRed
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構成内容 |
・anti-RFP rabbit polyclonal antibody ・Peroxidase anti-Rabbit IgG antibody ・control recombinant RFP protein ・Ultra Pure Tween-20 ・empty dropper bottle ・empty microfuge tubes ・Bovine Serum Albumin (BSA) ・Control Rabbit IgG ・FemtoMax Reagent A ・FemtoMax Reagent B ・Incubation box ・Instruction Manual.
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その他 |
[関連商品]600-401-379, 611-1322, FEMTOMAX-110, KCC003, 000-001-379, [Gene name]dsRed
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参考文献 |
[Pub Med ID]33574458
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Rockland Chemiluminescent Western Blot Kit for RFP contains sufficient reagents for up to 30 mini blots at 7.5 x 8 cm2.
This product is assembled as a kit. See attached protocol or CofA for further details.
RFP Western Blot: Known amounts of recombinant RFP and GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4C prior to probing the blot with the anti-GFP monoclonal antibody (p/n 600-301-215) diluted 1:1,000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4C. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
Flow Diagram for RFP Chemiluminescent Western Blot Procedure
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Rockland Chemiluminescent Western Blot Kit for RFP contains sufficient reagents for up to 30 mini blots at 7.5 x 8 cm2.
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メーカー |
品番 |
包装 |
RKL
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KCA379
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1 EACH
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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