RFP Chemiluminescent Western Blot Kit  

RFP Western Blot: Known amounts of recombinant RFP and GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody (p/n 600-301-215) diluted 1:1,000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4°C. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).