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Western Blot of anti-GFP monoclonal antibody. Lane 1: 64pg of recombinant GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364). Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4°C. Block: TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.
This product is assembled as a kit. See attached protocol or CofA for further details.
Flow Diagram for Chemiluminescent Western Blot Procedure. GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of Rockland's optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for GFP combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing GFP and its variants. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
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Western Blot of anti-GFP monoclonal antibody. Lane 1: 64pg of recombinant GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364). Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4°C. Block: TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.
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| 別品名 |
GFP Western Blotting Kit, GFP Chemiluminescent Kit, Green Fluorescent Protein Antibody Kit, Immunoblotting Kit, GFP, rGFP, eGFP
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| 適用 |
Western Blot
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| クローン |
9F9.F9
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| 標識物 |
Unlabeled
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| 構成内容 |
・anti-GFP monoclonal antibody
・Peroxidase anti-Mouse IgG antibody
・control recombinant GFP protein
・Ultra Pure Tween-20
・empty dropper bottle
・empty microfuge tubes
・Bovine Serum Albumin (BSA)
・Control Mouse IgG
・FemtoMax Reagent A
・FemtoMax Reagent B
・Incubation box
・Instruction Manual.
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| その他 |
[関連商品]600-301-215, 000-001-215, 610-1319, FEMTOMAX-110, KCC002, 600-301-215-0.1
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| 参考文献 |
Antibodies, A Laboratory Manuel. Ed Harlow and David Lane, eds. Cold Spring Harbor Press. 1988. Chapter 12 gives an excellent overview of Western Blotting techniques, including India Ink staining. Current protocols in Molecular Biology. J. Ausebel, et al, eds. John Wiley and Sons, New York. Gives a complete protocol of Western Blotting and Dot Blotting. Molecular Cloning: A Laboratory Manuel. 2nd Edition. J. Sambrook, E.F. Fritsch and T. Maniatis, eds. Cold Spring Harbor Press, 1989. Chapter 18 gives detailed protocols for both the production of cell lysates and electrophoresis and blotting of proteins. Antibodies, A Practical Approach. 2nd Edition. Catty, D., ed. IRL Press, Oxford, England. 1990. Volumes I and II represent a detailed and complete reference for most current antibody techniques.
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| メーカー |
品番 |
包装 |
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RKL
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KCA215
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1 KIT
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