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Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red (Red).
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Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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| 別品名 |
Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, MGC88685, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, G3PDH, OCAS, p38 component, Glyceraldehyde 3 phosphate dehydrogenase, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, OCT1 coactivator in S phase, 38-KD component, OTTHuMP00000174431, OTTHuMP00000174432, wu:fb33a10
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
AT8G4
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| 抗体クラス |
IgG2bκ
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| 抗原部位 |
a.a.1-335
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| Accession No.(Gene/Protein) |
NP_002037, P04406
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| 概要 |
Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.
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| 参考文献 |
Chuang DM, et al. (2005) Annu Rev Pharmacol Toxicol, 45:269-90.
Mazzola JL, et al. (2002) Neurotoxicology, 23(4-5):603-9.
Sirover MA. (1997) J Cell Biochem, 66(2):133-40.
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| メーカー |
品番 |
包装 |
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ATG
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ATGA0181
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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