適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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形状 |
液状
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参考文献 |
[Pub Med ID]22003197, 23518713, 23699174, 24069189, 25252997, 25605866, 26811382, 26966635, 28412251, 29727638, 29925665, 30154105, 31632969, 31849990, 32333091, 32660129, 32954498, 32967078, 33746979, 32310175+他多数
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Western Blot of anti GST tag antibody. Lane 1: Recombinant GST tagged recombinant protein 5 ug. Lane 2: Recombinant GST tagged recombinant protein 2 ug. Lane 3: Recombinant GST tagged recombinant protein 1 ug. Lane 4: Recombinant GST tagged recombinant protein 500 ng. Lane 5: Recombinant GST tagged recombinant protein 250 ng. Lane 6: Recombinant GST tagged recombinant protein 100 ng. Lane 7: Recombinant GST tagged recombinant protein 50 ng. Primary antibody: anti GST antibody at 1:1000 for overnight at 4C. Secondary antibody: donkey secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 78 kDa.
Western blot detection using Chemiluminescent FemtoMaxTM HRP Substrate. rPARP1 domain detected at 11 kDa after 3 sec exposure using primary antibody rabbit anti-serum 1:500 overnight, at 4C. Secondary antibody Peroxidase Goat Anti‐Rabbit IgG Antibody at 1:40,000. All incubations were performed in Blocking Buffer for Fluorescent Western Blocking (p/n MB‐070).
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Western Blot of anti GST tag antibody. Lane 1: Recombinant GST tagged recombinant protein 5 ug. Lane 2: Recombinant GST tagged recombinant protein 2 ug. Lane 3: Recombinant GST tagged recombinant protein 1 ug. Lane 4: Recombinant GST tagged recombinant protein 500 ng. Lane 5: Recombinant GST tagged recombinant protein 250 ng. Lane 6: Recombinant GST tagged recombinant protein 100 ng. Lane 7: Recombinant GST tagged recombinant protein 50 ng. Primary antibody: anti GST antibody at 1:1000 for overnight at 4C. Secondary antibody: donkey secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 78 kDa.
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メーカー |
品番 |
包装 |
RKL
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FEMTOMAX-020
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20 ML [2 component system]
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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