| 別品名 |
BBA060 protein, Borrelia burgdorferi p27, Surface lipoprotein p27
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| 種由来 |
Borrelia burgdorferi
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Borrelia burgdorferi
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| GENE ID |
1194336
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| Accession No.(Gene/Protein) |
NP_0457331, O50951
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| Gene Symbol |
BB_A60
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| 形状 |
凍結乾燥品
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| その他 |
[関連商品]618-103-012
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| 参考文献 |
Reindl M, Redl B, Stoffler G. (1993) Isolation and analysis of a linear plasmid-located gene of Borrelia burgdorferi B29 encoding a 27 kDa surface lipoprotein (p27) and its overexpression in Escherichia coli. Mol Microbiol. 8(6):1115-24. Schulze RJ, and Zuckert WR (2006) Borrelia burgdorferi lipoproteins are secreted to the outer surface by default. Molecular Microbiology 59 (5), 1473 - 1484.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot showing detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc？ MP 4000 imaging system (Bio-Rad).
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Western blot showing detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc？ MP 4000 imaging system (Bio-Rad).
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| メーカー |
品番 |
包装 |
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RKL
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200-401-C30
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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