別品名 |
Borrelia burgdorferi CRASP-2
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種由来 |
Borrelia burgdorferi
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Borrelia burgdorferi
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GENE ID |
1194149
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Accession No.(Gene/Protein) |
NP_045500, O50665
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Gene Symbol |
cspZ, BB_H06
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形状 |
滅菌済み液状品
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その他 |
[関連商品]618-100-012
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参考文献 |
Rossmann E, Kitiratschky V, Hofmann H, Kraiczy P, Simon MM, and Wallich R. (2006) Borrelia burgdorferi Complement Regulator-Acquiring Surface Protein 1 of the Lyme Disease spirochetes is expressed in humans and induces antibody responses restricted to nondenatured structural determinants. Infection and Immunity, December 74:12, 7024-7028 Kraiczya P, Schreibera J, Skerkab C, Hauptb K, Bradea V, Wallich R, and Zipfel PF. (2008) Assessment of the regions within complement regulator-acquiring surface protein (CRASP)-2 of Borrelia burgdorferi required for interaction with host immune regulators FHL-1 and factor H. Proceedings IX. International Jena Symposium on Tick-borne Diseases (formerly IPS) March 15-17, 2007, International Journal of Medical Microbiology, 298:1, 268-271
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot showing detection of 0.1 μg of recombinant CRASP-2 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-2 fusion protein (arrow; expected MW = 67.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc? MP 4000 imaging system (Bio-Rad).
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Western Blot showing detection of 0.1 μg of recombinant CRASP-2 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-2 fusion protein (arrow; expected MW = 67.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc? MP 4000 imaging system (Bio-Rad).
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メーカー |
品番 |
包装 |
RKL
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200-401-C19S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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