| 別品名 |
Decorin-binding protein B, Borrelia burgdorferi DbpB
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| 種由来 |
Borrelia burgdorferi
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Borrelia burgdorferi
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| GENE ID |
1194341
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| Accession No.(Gene/Protein) |
NP_045698, O50918
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| Gene Symbol |
dbpB, BB_A25
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| 形状 |
滅菌済み液状品
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| その他 |
[関連商品]618-100-012
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| 参考文献 |
Hagman K.E., Yang X., Wikel S.K., Schoeler G.B., Caimano M.J., Radolf J.D., Norgard M.V. (2000) Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B. burgdorferi in ticks. Infect. Immun. 68:4759-4764 [PubMed: 10899883] [Abstract] Fischer JR, Parveen N, Magoun L, Leong JM. (2003) Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi, the Lyme disease spirochete. Proc Natl Acad Sci U S A. Jun 10;100(12):7307-12. Epub 2003 May 28.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot showing detection of 0.1 μg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc？ MP 4000 imaging system (Bio-Rad).
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Western blot showing detection of 0.1 μg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc？ MP 4000 imaging system (Bio-Rad).
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| メーカー |
品番 |
包装 |
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RKL
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200-401-C16S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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