Anti Flagellin

Western Blot of Anti Flagellin Antibody. Lane 1: Flagellin Control Protein (p/n 000 001 C14) Reduced [0.1ug]. Lane 2: Opal Prestained Molecular Weight Markers (p/n MB 210 0500). Lane 3: Flagellin Control Protein Non Reduced [0.1ug]. Primary Antibody: Anti Flagellin at 1.0ug/mL overnight at 4C. Secondary Antibody: Goat anti Rabbit IgG peroxidase (p/n 611 103 122) at 1:70,000 for 30 mins at RT. Block: BLockOut (p/n MB 073) for 1hrs at RT. Predicted MW: ~76.3kDa. Exposure: 2sec.

Western blot showing detection of 0.1 ug of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDocTM MP 4000 imaging system (Bio-Rad).