| 別品名 |
Outer surface protein A, Borrelia burgdorferi OspA
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| 種由来 |
Borrelia burgdorferi
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Borrelia burgdorferi
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| GENE ID |
1194357
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| Accession No.(Gene/Protein) |
NP_045688, P0CL66
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| Gene Symbol |
ospA, BB_A15
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| 形状 |
凍結乾燥品
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| その他 |
[関連商品]618-103-012
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| 参考文献 |
Salverda. M, and Van Der. L, : United States Patent Application (2020)
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Anti OspA Antibody. Lane 1: Opal Pre stained Molecular Weight Marker (p/n MB 210 0500). Lane 2: 10ug HeLa spiked 50ng OspA. Lane 3: 10ug HeLa spiked 20ng OspA. Lane 4: 10ug HeLa spiked 10ng OspA. Lane 5: 10ug HeLa Whole Cell Lysate (p/n W09 000 364). Lane 6: 10ug HeLa spiked 50ng OspB. Lane 7: 10ug HeLa spiked 50ng OspC. Primary Antibody: Anti OspA at 1:1000 overnight at 2 8C. Secondary Antibody: Goat Anti Rabbit IgG HRP (p/n 611 103 122) at 1:70,000 for 30 min at RT. Block: BlockOut Buffer (p/n MB 073). Predicted MW: ~28.1kDa OspA protein, 70.5kDa MBP OspA fusion. Observed MW: ~30kDa, ~75kDa.
Western blot showing detection of 0.1 ug of recombinant OspA protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDocTM MP 4000 imaging system (Bio-Rad).
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Western Blot of Anti OspA Antibody. Lane 1: Opal Pre stained Molecular Weight Marker (p/n MB 210 0500). Lane 2: 10ug HeLa spiked 50ng OspA. Lane 3: 10ug HeLa spiked 20ng OspA. Lane 4: 10ug HeLa spiked 10ng OspA. Lane 5: 10ug HeLa Whole Cell Lysate (p/n W09 000 364). Lane 6: 10ug HeLa spiked 50ng OspB. Lane 7: 10ug HeLa spiked 50ng OspC. Primary Antibody: Anti OspA at 1:1000 overnight at 2 8C. Secondary Antibody: Goat Anti Rabbit IgG HRP (p/n 611 103 122) at 1:70,000 for 30 min at RT. Block: BlockOut Buffer (p/n MB 073). Predicted MW: ~28.1kDa OspA protein, 70.5kDa MBP OspA fusion. Observed MW: ~30kDa, ~75kDa.
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| メーカー |
品番 |
包装 |
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RKL
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200-401-C13
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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