| 別品名 |
bba64, Borrelia burgdorferi p35
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| 種由来 |
Borrelia burgdorferi
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Borrelia burgdorferi
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| GENE ID |
1194146
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| Accession No.(Gene/Protein) |
NP_045522, O50687
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| Gene Symbol |
BB-H32, bba64
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| 形状 |
凍結乾燥品
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| その他 |
[関連商品]618-103-012
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| 参考文献 |
Gautam, A., Hathaway, M., McClain, N., Ramesh, G., Ramamoorthy, R. (2008). Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter. Microbiology 154: 275-285 Robert D. Gilmore Jr., Rebekah R. Howison, Gabrielle Dietrich, Toni G. Patton, Dawn R. Clifton, and James A. Carroll, (2010). The bba64 gene of Borrelia burgdorferi, the Lyme disease agent, is critical for mammalian infection via tick bite transmission. PNAS vol. 107 no. 16 7515-7520 Fikrig E, Barthold SW, Sun W, Feng W, Telford SR 3rd, Flavell RA. (1997). Borrelia burgdorferi p35 and p37 proteins, expressed in vivo, elicit protective immunity. Immunity 1997 May;6(5):531-9.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Rabbit anti p35 antibody. Lane 1: p35 recombinant protein 50 ng load. Lane 2: p35 recombinant protein 100 ng load. Lane 3: p35 recombinant protein 250 ng load. Lane 4: p35 recombinant protein 500 ng load. Primary antibody: p35 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB 070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for p35. Other band(s): p35 splice variants and isoforms.
Western blot showing detection of 0.1 ug of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein (arrow; expected MW: 69.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDocTM MP 4000 imaging system (Bio-Rad).
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Western Blot of Rabbit anti p35 antibody. Lane 1: p35 recombinant protein 50 ng load. Lane 2: p35 recombinant protein 100 ng load. Lane 3: p35 recombinant protein 250 ng load. Lane 4: p35 recombinant protein 500 ng load. Primary antibody: p35 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB 070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for p35. Other band(s): p35 splice variants and isoforms.
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| メーカー |
品番 |
包装 |
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RKL
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200-401-C12
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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