| 別品名 |
ErpN, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N
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| 種由来 |
Borrelia burgdorferi
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Borrelia burgdorferi
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| GENE ID |
1194664
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| Accession No.(Gene/Protein) |
NP_051416, H7C7N5
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| Gene Symbol |
erpN, BB_L39
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| 形状 |
凍結乾燥品
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| その他 |
[関連商品]618-100-012
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| 参考文献 |
Stevenson B., Tilly K, and Rosa PA (1996) A Family of Genes Located on Four Separate 32-Kilobase Circular Plasmids in Borrelia burgdorferi B31. J Bacteriology (178)12 3508-3516 Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. (1997) Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390:580-586
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot showing detection of 0.1 μg recombinant proteins in Western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein (arrow; 59.5 kDa expected MW). Lane 3: fusion protein (MBP-tagged) plus cleaved fusion proteins (without MBP). Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in lane 3 are fusion protein (top band), or breakdown products of the fusion protein (bands in middle of blot). Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc？ MP 4000 imaging system (Bio-Rad).
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Western blot showing detection of 0.1 μg recombinant proteins in Western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein (arrow; 59.5 kDa expected MW). Lane 3: fusion protein (MBP-tagged) plus cleaved fusion proteins (without MBP). Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in lane 3 are fusion protein (top band), or breakdown products of the fusion protein (bands in middle of blot). Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc？ MP 4000 imaging system (Bio-Rad).
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| メーカー |
品番 |
包装 |
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RKL
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200-401-C10
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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