Anti Esrp-1  

Anti-ESRP-1 by western blot shows detection of ESRP-1 seen in lane 2. Lane 1: GFP-transfected-293T cell extracts, Lane 2: ESRP-1-transfected-293T cell extracts, Lane 3: ESRP2-transfected 293T cell lysates. Briefly, each lane contains approximately 5 μg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

Protein expression levels of the splicing factors bound to the putative motifs in pressure-overload cardiac hypertrophy. (A) Heart weight/body weight ratio (HW/BW) at 1 week after Sham or TAC operation (left panel). The relative expression levels of the hypertrophic markers in the mouse models were determined by qRT-PCR (right panel). The differences are statistically significant at *p？< 0.05, **p？< 0.01 and ***p？< 0.001 (The student？t-test). Bars repre-sent means ± SD (n = 3). (B) The relative protein expression levels of the putative splicing factors bound to the identified intronic motifs were measured by Western blotting. Bars represent means ± SD (n = 3). The differences are statistically significant at *p？< 0.05, **p？< 0.01 (The student？t-test). Fig 3. PMID:？24552714