Anti p53, phospho Thr18  

Immunofluorescence analysis of rat-lung tissue. 1,p53 (phospho Thr18) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,p53 (phospho Thr18) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,p53 (phospho Thr18) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of customer's using Phospho-p53 (T18) Polyclonal Antibody. Antibody was diluted at 1:2000

Western Blot analysis of various cells using Phospho-p53 (T18) Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from Ovary cancer and K562 cells, using p53 (Phospho-Thr18) Antibody. The lane on the right is blocked with the phospho peptide.