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※サムネイル画像をクリックすると拡大画像が表示されます。
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL32 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate Lane 2.: U937 cell lysate
Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of IL32 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IL32 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL32 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate Lane 2.: U937 cell lysate
Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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| 別品名 |
Interleukin 32 isoform A, IL-32, Natural killer cells protein 4(NK4), Tumor necrosis factor alpha-inducing factor (TAIF), Interleukin 32, Interleukin 32 isoform A IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, Interleukin 32, Natural killer cells protein 4, NK 4, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, il32
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immuno Fluorescence Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
AT2F9
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| 抗体クラス |
IgG1κ
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| 抗原部位 |
a.a.1-131
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| Accession No.(Gene/Protein) |
NP_001012651, P24001
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| 概要 |
The gene encoding IL32 is located on human chromosome 16p13. 3 and is organized into eight exons. IL32 is mainly expressed in natural killer cells, T cells, epithelial cells, and blood monocytes. It can induce the proinflammatory cytokines TNFalpha and IL1beta in murine peritoneal macrophages as well as in phorbol ester-differentiated human THP1 cells. In the inflamed lesions of chronic pancreas, the ductal expression of IL32 was markedly increased. Pancreatic duct cells are the local source of IL32, and IL32 may play an important role in inflammatory responses and pancreatic cancer growth. Recently, a synergism between IL32 and other well characterized players in innate immunity has been documented.
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| 参考文献 |
Nishida A, et al. (2009) J Biol Chem, 284(26):17868-76. Kobayashi H, et al. (2009) Cytokine, 46(3):351-8. Mabilleau G, et al. (2009) PLoS One, 4(1):e4173.
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| メーカー |
品番 |
包装 |
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ATG
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ATGA0170
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50 UL [1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-70℃
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