Anti VDAC/Porin  

Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 μg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800^TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.

Western Blot of Rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 μg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at RT. Predicted/Observed size: 32 kDa, 32 kDa for VDAC/Porin.

Western Blot of Rabbit anti-VDAC/Porin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: LNCaP Lysate (p/n W09-001-GJ9). Lane 7: A-172 Lysate (p/n W09-001-GL5). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 μg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 31 kDa for VDAC\Porin.

HSV-1 infection of HaCaT cells upon silencing of Rac1, Cdc42, or RhoA. HaCaT cells were transfected with Rac1-siRNA, Cdc42-siRNA, RhoA-siRNA, or nonspecific control siRNAs, followed by infection with HSV-1 (20 PFU/cell) at 72 h posttransfection. (A) Total protein extracts were prepared, and proteins were resolved on SDS-PAGE gels (15%). Expression of the Rho GTPases was analyzed with mouse anti-Rac1, mouse anti-Cdc42, or mouse anti-RhoA antibodies. Staining of PDI or VDAC was used as a loading control.？Fig 4. PMID:？19640983

(B) Immunoblot analysis of myelin-enriched fractions and equal amounts of brain lysate to compare the abundance of marker proteins for compact myelin (PLP/DM20, MBP), non-compact myelin (CNP, SIRT2), the oligodendroglial nuclear/cytoplasmic compartment (OLIG2), astrocytes (GFAP), microglia (AIF1/IBA1), neuronal plasma membrane (GPM6A), axonal microtubules (TUBB3/TUJ1), and mitochondria (VDAC). Blot represents three biological replicates (male c57Bl6/N mice, age P75). Note that myelin markers were enriched in purified myelin while markers of other cellular sources were reduced.？Fig 1. PMID:？27173133

？(B) TPP-IOA (1？μM) decreases VDAC protein levels. Top inset depicts representative VDAC (~？32？kDa) and β-actin (~？43？kDa) band intensities. In A&B, measurements were made with lysates prepared from SH-SY5Y cells harvested immediately after 3？h of exposure to 0.1% DMSO (Vehicle control) or 1？μM TPP-IOA. Bars represent means + SEM from two or three experimental replicates. *？=？p-value？<？0.05 (Student's？t-test). Fig 10. ？PMID:？27836699

MB？ablation alters metabolism in？p53172H/H？mice. Male mice of the indicated genotypes without evidence of tumor were used for the following experiments.？A,？Immunoblot of indicated proteins in skeletal muscle of 10 wk old mice. Actinin and VDAC serve as protein loading controls.？Fig 1. PMID:？32958587