Anti VDAC/Porin  

Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 μg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.

Western Blot of Rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 μg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at RT. Predicted/Observed size: 32 kDa, 32 kDa for VDAC/Porin.

Western Blot of Rabbit anti-VDAC/Porin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: LNCaP Lysate (p/n W09-001-GJ9). Lane 7: A-172 Lysate (p/n W09-001-GL5). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 μg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 31 kDa for VDAC\Porin.