別品名 |
anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
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標識物 |
DyLightTM 649
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Dot Blot
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
33D10.D2
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Tag情報 |
6X HIS
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形状 |
凍結乾燥品
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参考文献 |
[Pub Med ID]25242553, 32938950, 27893463, 34216984, 33846527
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA of Mouse anti 6xHIS Tag Antibody. Antigen: HIS tagged purified protein and E. coli cell lysates expressing HIS Tagged construct, GST and RON tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2 fold. Mid point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE 1000).
Properties of DyLightTM Conjugates.
DyLightTM dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLightTM 549 conjugate. Anti-TNFα was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
DyLight? dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight? 549 conjugate. Anti-TNF? was detected using a DyLight? 649 conjugate. The image was captured using the Typhoon? 9410 Imaging System.
Western Blot of Mouse Anti-6X HIS EPITOPE TAG Antibody DyLightTM 649 Conjugated. Lane 1: Molecular Weight Ladder. Lane 2: Recombinant 6xHIS-SUMO-GFP. Lane 3: Nag1. Load: 100ng Primary antibody: 6X HIS EPITOPE TAG DyLightTM 649 Conjugated at 1:1000 30 min RT. Block: 1% BSA-TTBS 30 min RT. Observed size: Lane 2: 45 kDa, Lane 3: 16 and 32 kDa. Other band(s): Lysate breakdown.
Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
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ELISA of Mouse anti 6xHIS Tag Antibody. Antigen: HIS tagged purified protein and E. coli cell lysates expressing HIS Tagged construct, GST and RON tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2 fold. Mid point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE 1000).
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メーカー |
品番 |
包装 |
RKL
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200-343-382
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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