別品名 |
anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
|
標識物 |
DyLightTM 549
|
精製度 |
Ig fraction - Protein A
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay
|
免疫動物 |
Mouse
|
抗体クラス |
IgG1κ
|
クローン |
33D10.D2
|
Tag情報 |
6X HIS
|
形状 |
凍結乾燥品
|
参考文献 |
Brandsma, M ; X Wang, H Diao, SE. Kohalmi1, AM Jevnikar and S. Ma (2009) A Proficient Approach to the Production of Therapeutic Glucagon-Like Peptide-1 (GLP-1) in Transgenic PlantsThe Open Biotechnology Journal, 3, 57-66 57 1874-0707/09
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
※サムネイル画像をクリックすると拡大画像が表示されます。
DyLightTM dyes can be used for two color Western Blot detection with low background and high signal. Anti tubulin was detected using a DyLightTM 549 conjugate. Anti TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).
Properties of DyLight? Conjugates.
ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).
Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
|
|
DyLightTM dyes can be used for two color Western Blot detection with low background and high signal. Anti tubulin was detected using a DyLightTM 549 conjugate. Anti TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
|
|
|
メーカー |
品番 |
包装 |
RKL
|
200-342-382
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
法規制 |
毒
|
保存温度 |
4℃
|
|