別品名 |
anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
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標識物 |
DyLightTM 488
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
33D10.D2
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Tag情報 |
6X HIS
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形状 |
凍結乾燥品
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参考文献 |
Brandsma, M ; X Wang, H Diao, SE. Kohalmi1, AM Jevnikar and S. Ma (2009) A Proficient Approach to the Production of Therapeutic Glucagon-Like Peptide-1 (GLP-1) in Transgenic PlantsThe Open Biotechnology Journal, 3, 57-66 57 1874-0707/09
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA of Mouse anti 6xHIS Tag Antibody. Antigen: HIS tagged purified protein and E. coli cell lysates expressing HIS Tagged construct, GST and RON tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2 fold. Mid point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE 1000).
Properties of DyLightTM Fluorescent Dyes.
DyLight? dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight? dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight? 488 conjugate (green). Anti-Tubulin was detected using a DyLight? 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
Properties of DyLight? Fluorescent Dyes.
Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
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ELISA of Mouse anti 6xHIS Tag Antibody. Antigen: HIS tagged purified protein and E. coli cell lysates expressing HIS Tagged construct, GST and RON tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2 fold. Mid point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE 1000).
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メーカー |
品番 |
包装 |
RKL
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200-341-382
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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