別品名 |
anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
33D10.D2.G8
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Tag情報 |
6X HIS
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]28893174, 32205407, 33208520, 24126912, 34936880
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Characterization of purity and antigenicity of CKAP4 constructs. a SDS PAGE of normalized amounts of purified CKAP4 constructs followed by silver staining. bf Western blots using anti His tag, anti CKAP4 (G1/296), anti CKAP4 (epitope region: 216265), anti CKAP4 (epitope region: 375425), and anti CKAP4 (epitope region: 552602) primary antibody, respectively. Separate blots were used for each antibody. Molecular weight standards are indicated on left and right Figure provided by CiteAb. Source: BMC Biochem, PMID: 28893174.
ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).
Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
Western Blot of Mouse Anti-6X HIS Epitope Tag Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: rec EGF -C-term HIS tag [0.02ug] [+]. Lane 3: rec Luciferase N-term HIS tag [0.10ug] [+]. Lane 4: Zic1 - C-myc/DDK Tag [0.10ug] [-]. Primary Antibody: Mouse Anti-6X HIS at 1.0ug/mL overnight at 2-8C. Secondary Antibody: Rabbit Anti-Mouse HRP (p/n 610-403-C46) at 1:40,000 for 30mins at RT. Block: BlockOut Buffer (p/m MB-073) for 1hr at RT. Bands are recognized at the expected MW. Exposure: 2sec.
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Characterization of purity and antigenicity of CKAP4 constructs. a SDS PAGE of normalized amounts of purified CKAP4 constructs followed by silver staining. bf Western blots using anti His tag, anti CKAP4 (G1/296), anti CKAP4 (epitope region: 216265), anti CKAP4 (epitope region: 375425), and anti CKAP4 (epitope region: 552602) primary antibody, respectively. Separate blots were used for each antibody. Molecular weight standards are indicated on left and right Figure provided by CiteAb. Source: BMC Biochem, PMID: 28893174.
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メーカー |
品番 |
包装 |
RKL
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200-301-382
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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