| 別品名 |
GST, Glutathione-S-Transferase
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| Tag情報 |
GST
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]23817741, 29507658
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Rabbit Anti GST Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: rec. GST/HeLa Whole Cell Lysate (0.1ug/10ug). Lane 3: rec. GST/HeLa Whole Cell Lysate (0.05ug/10ug). Lane 4: rec. GST/HeLa Whole Cell Lysate (0.03ug/10ug). Lane 5: HeLa Whole Cell Lysate [p/n W09 000 364] (10ug). Lane 6: 12 Epitope GST Lysate [p/n MB 302 0100] (5ug). Primary Antibody: Anti GST at 1.0ug/mL overnight at 2 8C. Secondary Antibody: Goat Anti Rabbit IgG HRP (p/n 611 103 122) at 1:70,000 for 30mins at RT. Blocking: 1% Casein/1XTBS/0.05%Tween. Predicted MW: GST 26kDa, 12 Epitope GST Tag Protein Marker Lysate 48kDa.
Western Blot showing detection of recombinant GST protein (0.25 ug) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDocTM MP 4000 imaging system (Bio-Rad).
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Western Blot of Rabbit Anti GST Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: rec. GST/HeLa Whole Cell Lysate (0.1ug/10ug). Lane 3: rec. GST/HeLa Whole Cell Lysate (0.05ug/10ug). Lane 4: rec. GST/HeLa Whole Cell Lysate (0.03ug/10ug). Lane 5: HeLa Whole Cell Lysate [p/n W09 000 364] (10ug). Lane 6: 12 Epitope GST Lysate [p/n MB 302 0100] (5ug). Primary Antibody: Anti GST at 1.0ug/mL overnight at 2 8C. Secondary Antibody: Goat Anti Rabbit IgG HRP (p/n 611 103 122) at 1:70,000 for 30mins at RT. Blocking: 1% Casein/1XTBS/0.05%Tween. Predicted MW: GST 26kDa, 12 Epitope GST Tag Protein Marker Lysate 48kDa.
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| メーカー |
品番 |
包装 |
|
RKL
|
600-401-B86
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1 MG
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
４℃
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