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Western blot using Rockland's affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T transfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1μg/mL and incubated overnight at 4°C. ECL detection was used.
RRM2B silencing results in dysfunctional oxidative phosphorylation and enhanced ROS production. B: Western blot analysis shows reduced expression of the core genes of the oxidative phosphorylation (OXPHOS) complex II and III in RRM2B-silenced cells.？ Fig 3. PMID:？30662615
RRM2B and CypD are inversely correlated in protein expression levels as well as in their associated clinical pathologic parameters. A: Representative immunohistochemistry (IHC) image from two breast cancer patients (Patient 1, Patient 2). Fig 5. PMID:？30662615
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Western blot using Rockland's affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T transfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1μg/mL and incubated overnight at 4°C. ECL detection was used.
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| 別品名 |
rabbit anti-RRM2B p53R2 antibody, rabbit anti-RRM2B antibody, rabbit anti-p53R2 antibody, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein
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| 別包装 |
あり
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Serum
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| GENE ID |
50484
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| Accession No.(Gene/Protein) |
42544136, Q7LG56
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| Gene Symbol |
RRM2B
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]26675256,30662615
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-B67S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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