Anti GST

Western Blot of Rabbit Anti GST Antibody. Lane 1: MW markers. Lane 2: recombinant GST protein (p/n 000 001 200). Load: 0.25 ug. Primary Antibody: Anti GST at 1:1000 at room temperature for 30 min. Secondary Antibody: HRP conjugated Goat Anti Rabbit (p/n 611 103 122) secondary antibody was used at 1:40,000 in blocking buffer (p/n MB 070). Blocking: 1% BSA TTBS (p/n MB 013, diluted to 1X) overnight at 4C.

Western Blot of Rabbit anti-GST antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383). Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein (p/n 000-001-200). Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: GST antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26 kDa for GST.